human syndecan 4 apc conjugated antibody Search Results


anti sdc4 antibody  (R&D Systems)
94
R&D Systems anti sdc4 antibody
<t>SDC4</t> contributes to AAV9’s entry into hCMEC/D3 cells. ( A – F ) Imaging flow cytometry assessment of SDC4 expression and AAV9-mediated gene (GFP) delivery in wild-type (WT) and SDC4 KD hCMEC/D3 cells. SDC4 KD was performed using SDC4-specific shRNA plasmids. WT and SDC4 KD hCMEC/D3 cells were treated 4 × 10 4 vg/cell AAV9-GFP for 72 h at 37 °C. SDC4 and GFP expression levels were measured with imaging flow cytometry, as shown by the representative histograms and cellular images of three independent experiments. Scale bar = 20 μm. The effect of SDC4 KD on SDC4 and GFP expression expressed as percent inhibition. The bars represent the mean ± SEM of three independent experiments. Statistical significance vs. WT was assessed with analysis of variance (ANOVA). ** p < 0.01; *** p < 0.001. ( G ) SDS-PAGE showing VP1-3 immunoprecipitated with SDC4 from AAV9-treated (4 × 10 4 vg/cell AAv9 for 6 h at 37 °C) hCMEC/D3 cells’ extracts. Lane 1: 10 6 vg recombinant AAV9; lane 2–3: immunoprecipitates of AAV9-treated WT hCMEC/D3 and SDC4 KD cells; lane 4: MW marker; lane 5–6: immunoprecipitates of WT and SDC4 KD hCMEC/D3 cells untreated with AAV9 (i.e., controls). Standard protein size markers are indicated on the right.
Anti Sdc4 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+syndecan+4+apc+conjugated+antibody/Human+Syndecan-4+APC-conjugated+Antibody/pmc09963952-148-24-26
Average 94 stars, based on 1 article reviews
anti sdc4 antibody - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
allophycocyanin apc conjugated rat monoclonal anti human syndecan 1 anti hspg  (R&D Systems)
94
R&D Systems allophycocyanin apc conjugated rat monoclonal anti human syndecan 1 anti hspg
<t>SDC4</t> contributes to AAV9’s entry into hCMEC/D3 cells. ( A – F ) Imaging flow cytometry assessment of SDC4 expression and AAV9-mediated gene (GFP) delivery in wild-type (WT) and SDC4 KD hCMEC/D3 cells. SDC4 KD was performed using SDC4-specific shRNA plasmids. WT and SDC4 KD hCMEC/D3 cells were treated 4 × 10 4 vg/cell AAV9-GFP for 72 h at 37 °C. SDC4 and GFP expression levels were measured with imaging flow cytometry, as shown by the representative histograms and cellular images of three independent experiments. Scale bar = 20 μm. The effect of SDC4 KD on SDC4 and GFP expression expressed as percent inhibition. The bars represent the mean ± SEM of three independent experiments. Statistical significance vs. WT was assessed with analysis of variance (ANOVA). ** p < 0.01; *** p < 0.001. ( G ) SDS-PAGE showing VP1-3 immunoprecipitated with SDC4 from AAV9-treated (4 × 10 4 vg/cell AAv9 for 6 h at 37 °C) hCMEC/D3 cells’ extracts. Lane 1: 10 6 vg recombinant AAV9; lane 2–3: immunoprecipitates of AAV9-treated WT hCMEC/D3 and SDC4 KD cells; lane 4: MW marker; lane 5–6: immunoprecipitates of WT and SDC4 KD hCMEC/D3 cells untreated with AAV9 (i.e., controls). Standard protein size markers are indicated on the right.
Allophycocyanin Apc Conjugated Rat Monoclonal Anti Human Syndecan 1 Anti Hspg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+syndecan+4+apc+conjugated+antibody/Human+Syndecan-4+APC-conjugated+Antibody/pm29256602__ja7b08518_si_001-16-0-16
Average 94 stars, based on 1 article reviews
allophycocyanin apc conjugated rat monoclonal anti human syndecan 1 anti hspg - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

Image Search Results


SDC4 contributes to AAV9’s entry into hCMEC/D3 cells. ( A – F ) Imaging flow cytometry assessment of SDC4 expression and AAV9-mediated gene (GFP) delivery in wild-type (WT) and SDC4 KD hCMEC/D3 cells. SDC4 KD was performed using SDC4-specific shRNA plasmids. WT and SDC4 KD hCMEC/D3 cells were treated 4 × 10 4 vg/cell AAV9-GFP for 72 h at 37 °C. SDC4 and GFP expression levels were measured with imaging flow cytometry, as shown by the representative histograms and cellular images of three independent experiments. Scale bar = 20 μm. The effect of SDC4 KD on SDC4 and GFP expression expressed as percent inhibition. The bars represent the mean ± SEM of three independent experiments. Statistical significance vs. WT was assessed with analysis of variance (ANOVA). ** p < 0.01; *** p < 0.001. ( G ) SDS-PAGE showing VP1-3 immunoprecipitated with SDC4 from AAV9-treated (4 × 10 4 vg/cell AAv9 for 6 h at 37 °C) hCMEC/D3 cells’ extracts. Lane 1: 10 6 vg recombinant AAV9; lane 2–3: immunoprecipitates of AAV9-treated WT hCMEC/D3 and SDC4 KD cells; lane 4: MW marker; lane 5–6: immunoprecipitates of WT and SDC4 KD hCMEC/D3 cells untreated with AAV9 (i.e., controls). Standard protein size markers are indicated on the right.

Journal: International Journal of Molecular Sciences

Article Title: Syndecan-4 Mediates the Cellular Entry of Adeno-Associated Virus 9

doi: 10.3390/ijms24043141

Figure Lengend Snippet: SDC4 contributes to AAV9’s entry into hCMEC/D3 cells. ( A – F ) Imaging flow cytometry assessment of SDC4 expression and AAV9-mediated gene (GFP) delivery in wild-type (WT) and SDC4 KD hCMEC/D3 cells. SDC4 KD was performed using SDC4-specific shRNA plasmids. WT and SDC4 KD hCMEC/D3 cells were treated 4 × 10 4 vg/cell AAV9-GFP for 72 h at 37 °C. SDC4 and GFP expression levels were measured with imaging flow cytometry, as shown by the representative histograms and cellular images of three independent experiments. Scale bar = 20 μm. The effect of SDC4 KD on SDC4 and GFP expression expressed as percent inhibition. The bars represent the mean ± SEM of three independent experiments. Statistical significance vs. WT was assessed with analysis of variance (ANOVA). ** p < 0.01; *** p < 0.001. ( G ) SDS-PAGE showing VP1-3 immunoprecipitated with SDC4 from AAV9-treated (4 × 10 4 vg/cell AAv9 for 6 h at 37 °C) hCMEC/D3 cells’ extracts. Lane 1: 10 6 vg recombinant AAV9; lane 2–3: immunoprecipitates of AAV9-treated WT hCMEC/D3 and SDC4 KD cells; lane 4: MW marker; lane 5–6: immunoprecipitates of WT and SDC4 KD hCMEC/D3 cells untreated with AAV9 (i.e., controls). Standard protein size markers are indicated on the right.

Article Snippet: Stable KD cells were selected in 2 mg G418 and sorted using imaging flow cytometry (Amnis® FlowSight®, Luminex Corporation, Austin, TX, USA) with APC-conjugated anti-SDC4 antibody (RnD Systems, Minneapolis, MN, USA, cat. no. FAB29181A, 5:100 dilution) and respective isotype control (rat IgG2A APC isotype control, RnD Systems, cat. no. IC006A, 5:100).

Techniques: Imaging, Flow Cytometry, Expressing, shRNA, Inhibition, SDS Page, Immunoprecipitation, Recombinant, Marker

AAV9 colocalizes with SDC4 during cellular internalization. hCMEC/D3 cells were incubated with AAV9 (4 × 10 4 vg/cell) for 6 h at 37 °C. The cells were then trypsinized, fixed, permeabilized and treated with AF488-labeled AAV9 (green) and APC-labeled SDC4 (red) antibodies. AAV9’s colocalization with SDC4 was analyzed with confocal microscopy. Representative images of three independent experiments are shown. Scale bar = 10 μm. The MOC ± SEM and PCC ± SEM for the overlap and colocalization of SDC4 with AAV9 are indicated below the images.

Journal: International Journal of Molecular Sciences

Article Title: Syndecan-4 Mediates the Cellular Entry of Adeno-Associated Virus 9

doi: 10.3390/ijms24043141

Figure Lengend Snippet: AAV9 colocalizes with SDC4 during cellular internalization. hCMEC/D3 cells were incubated with AAV9 (4 × 10 4 vg/cell) for 6 h at 37 °C. The cells were then trypsinized, fixed, permeabilized and treated with AF488-labeled AAV9 (green) and APC-labeled SDC4 (red) antibodies. AAV9’s colocalization with SDC4 was analyzed with confocal microscopy. Representative images of three independent experiments are shown. Scale bar = 10 μm. The MOC ± SEM and PCC ± SEM for the overlap and colocalization of SDC4 with AAV9 are indicated below the images.

Article Snippet: Stable KD cells were selected in 2 mg G418 and sorted using imaging flow cytometry (Amnis® FlowSight®, Luminex Corporation, Austin, TX, USA) with APC-conjugated anti-SDC4 antibody (RnD Systems, Minneapolis, MN, USA, cat. no. FAB29181A, 5:100 dilution) and respective isotype control (rat IgG2A APC isotype control, RnD Systems, cat. no. IC006A, 5:100).

Techniques: Incubation, Labeling, Confocal Microscopy

Contribution of the various parts of the SDC4 ectodomain to AAV9 uptake. GFP-tagged SDC4 mutants incubated with AAV9 vectors at 4 × 10 4 vg/cell for 6 h were fixed, permeabilized, and treated with specific primary AAV9 and AF 633-labeled secondary antibodies. The cellular uptake of AAV9 was then analyzed with imaging flow cytometry and confocal microscopy. ( A , B ) Representative fluorescent images and flow cytometry histograms showing the intracellular fluorescence of AAV9-treated SDC4 mutants. Scale bar = 20 μm. The indicated BDS values of AAV9 and SDCs represent the mean + SEM of eight independent experiments. Statistical significance was assessed with ANOVA. ( C ) Detected fluorescence intensities were normalized to AAV9-treated transfectants expressing GFP-labeled WT SDC4 as standards. The bars represent mean + SEM of eight independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05; *** p < 0.001. ( D ) Confocal microscopic visualization of AAV9-treated SDC4 mutants. Scale bar = 10 μm. MOC ± SEM for the overlap of AAV9 with the mutants was calculated by analysis of 15 images with ~10 cells in each image (from three separate samples). Statistical significance vs. AAV9-treated transfectants expressing WT SDC4 (standards) was assessed with ANOVA. *** p < 0.001.

Journal: International Journal of Molecular Sciences

Article Title: Syndecan-4 Mediates the Cellular Entry of Adeno-Associated Virus 9

doi: 10.3390/ijms24043141

Figure Lengend Snippet: Contribution of the various parts of the SDC4 ectodomain to AAV9 uptake. GFP-tagged SDC4 mutants incubated with AAV9 vectors at 4 × 10 4 vg/cell for 6 h were fixed, permeabilized, and treated with specific primary AAV9 and AF 633-labeled secondary antibodies. The cellular uptake of AAV9 was then analyzed with imaging flow cytometry and confocal microscopy. ( A , B ) Representative fluorescent images and flow cytometry histograms showing the intracellular fluorescence of AAV9-treated SDC4 mutants. Scale bar = 20 μm. The indicated BDS values of AAV9 and SDCs represent the mean + SEM of eight independent experiments. Statistical significance was assessed with ANOVA. ( C ) Detected fluorescence intensities were normalized to AAV9-treated transfectants expressing GFP-labeled WT SDC4 as standards. The bars represent mean + SEM of eight independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05; *** p < 0.001. ( D ) Confocal microscopic visualization of AAV9-treated SDC4 mutants. Scale bar = 10 μm. MOC ± SEM for the overlap of AAV9 with the mutants was calculated by analysis of 15 images with ~10 cells in each image (from three separate samples). Statistical significance vs. AAV9-treated transfectants expressing WT SDC4 (standards) was assessed with ANOVA. *** p < 0.001.

Article Snippet: Stable KD cells were selected in 2 mg G418 and sorted using imaging flow cytometry (Amnis® FlowSight®, Luminex Corporation, Austin, TX, USA) with APC-conjugated anti-SDC4 antibody (RnD Systems, Minneapolis, MN, USA, cat. no. FAB29181A, 5:100 dilution) and respective isotype control (rat IgG2A APC isotype control, RnD Systems, cat. no. IC006A, 5:100).

Techniques: Incubation, Labeling, Imaging, Flow Cytometry, Confocal Microscopy, Fluorescence, Expressing

The effect of undersulfation on SDC4 expression and AAV9-mediated gene transduction. Stable SDC4 transfectants (created in K562 cells) were preincubated with or without NaClO 3 for 48 h. Effect of NaClO 3 preincubation on HS and SDC4 expression was measured with imaging flow cytometry by incubating the cells with HS- and SDC4-specific antibodies. SDC4 transfectants preincubated with or without NaClO 3 were then treated with AAV9-GFP vectors at 4 × 10 4 vg/cell. After 72 h of incubation with AAV9-GFP, GFP expression was measured with imaging flow cytometry. ( A – D ) Representative flow cytometry histograms and fluorescent cellular images showing HS, GFP, and SDC4 expression of cells preincubated with or without NaClO 3 . Scale bar = 20 μm. ( E ) Detected HS, GFP, and SDC4 expression levels in SDC4 transfectants were normalized to those untreated with NaClO 3 (controls). The bars represent the mean + SEM of six independent experiments. Statistical significance was assessed with ANOVA. *** p < 0.001. ( F ) Linear regression between SDC4 expression and AAV9-mediated GFP transduction.

Journal: International Journal of Molecular Sciences

Article Title: Syndecan-4 Mediates the Cellular Entry of Adeno-Associated Virus 9

doi: 10.3390/ijms24043141

Figure Lengend Snippet: The effect of undersulfation on SDC4 expression and AAV9-mediated gene transduction. Stable SDC4 transfectants (created in K562 cells) were preincubated with or without NaClO 3 for 48 h. Effect of NaClO 3 preincubation on HS and SDC4 expression was measured with imaging flow cytometry by incubating the cells with HS- and SDC4-specific antibodies. SDC4 transfectants preincubated with or without NaClO 3 were then treated with AAV9-GFP vectors at 4 × 10 4 vg/cell. After 72 h of incubation with AAV9-GFP, GFP expression was measured with imaging flow cytometry. ( A – D ) Representative flow cytometry histograms and fluorescent cellular images showing HS, GFP, and SDC4 expression of cells preincubated with or without NaClO 3 . Scale bar = 20 μm. ( E ) Detected HS, GFP, and SDC4 expression levels in SDC4 transfectants were normalized to those untreated with NaClO 3 (controls). The bars represent the mean + SEM of six independent experiments. Statistical significance was assessed with ANOVA. *** p < 0.001. ( F ) Linear regression between SDC4 expression and AAV9-mediated GFP transduction.

Article Snippet: Stable KD cells were selected in 2 mg G418 and sorted using imaging flow cytometry (Amnis® FlowSight®, Luminex Corporation, Austin, TX, USA) with APC-conjugated anti-SDC4 antibody (RnD Systems, Minneapolis, MN, USA, cat. no. FAB29181A, 5:100 dilution) and respective isotype control (rat IgG2A APC isotype control, RnD Systems, cat. no. IC006A, 5:100).

Techniques: Expressing, Transduction, Imaging, Flow Cytometry, Incubation

The AAV9 interactome in SDC4 transfectants.

Journal: International Journal of Molecular Sciences

Article Title: Syndecan-4 Mediates the Cellular Entry of Adeno-Associated Virus 9

doi: 10.3390/ijms24043141

Figure Lengend Snippet: The AAV9 interactome in SDC4 transfectants.

Article Snippet: Stable KD cells were selected in 2 mg G418 and sorted using imaging flow cytometry (Amnis® FlowSight®, Luminex Corporation, Austin, TX, USA) with APC-conjugated anti-SDC4 antibody (RnD Systems, Minneapolis, MN, USA, cat. no. FAB29181A, 5:100 dilution) and respective isotype control (rat IgG2A APC isotype control, RnD Systems, cat. no. IC006A, 5:100).

Techniques: Membrane, Variant Assay

SDC4 overexpression increases AAV9-mediated GFP transduction in SH-SY5Y cells. SDC4 transfectants (created in SH-SY5Y cells) and WT SH-SY5Y cells were incubated with AAV9-GFP (4 × 10 4 vg/cell) at 37 °C for 72 h. GFP expression was then analyzed with imaging flow cytometry. ( A – C ) Representative flow cytometry histograms and fluorescent images showing the GFP and SDC4 expression levels in WT SH-SY5Y cells and SDC4 transfectants treated with AAV9-GFP. Scale bar = 20 μm. ( D , E ) Detected SDC4 and GFP expression levels of AAV9-treated SDC4 transfectants were normalized to that of WT SH-SY5Y cells as standards. The bars represent the mean + SEM of three independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05; ** p < 0.01.

Journal: International Journal of Molecular Sciences

Article Title: Syndecan-4 Mediates the Cellular Entry of Adeno-Associated Virus 9

doi: 10.3390/ijms24043141

Figure Lengend Snippet: SDC4 overexpression increases AAV9-mediated GFP transduction in SH-SY5Y cells. SDC4 transfectants (created in SH-SY5Y cells) and WT SH-SY5Y cells were incubated with AAV9-GFP (4 × 10 4 vg/cell) at 37 °C for 72 h. GFP expression was then analyzed with imaging flow cytometry. ( A – C ) Representative flow cytometry histograms and fluorescent images showing the GFP and SDC4 expression levels in WT SH-SY5Y cells and SDC4 transfectants treated with AAV9-GFP. Scale bar = 20 μm. ( D , E ) Detected SDC4 and GFP expression levels of AAV9-treated SDC4 transfectants were normalized to that of WT SH-SY5Y cells as standards. The bars represent the mean + SEM of three independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05; ** p < 0.01.

Article Snippet: Stable KD cells were selected in 2 mg G418 and sorted using imaging flow cytometry (Amnis® FlowSight®, Luminex Corporation, Austin, TX, USA) with APC-conjugated anti-SDC4 antibody (RnD Systems, Minneapolis, MN, USA, cat. no. FAB29181A, 5:100 dilution) and respective isotype control (rat IgG2A APC isotype control, RnD Systems, cat. no. IC006A, 5:100).

Techniques: Over Expression, Transduction, Incubation, Expressing, Imaging, Flow Cytometry

AAV9-mediated GFP transduction is more efficient in U-87 MG than in SH-SY5Y cells. WT U-87 MG and SH-SY5Y cells were incubated with AAV9-GFP (4 × 10 4 vg/cell) for 72 h. GFP expression was then analyzed with imaging flow cytometry. ( A – C ) Representative flow cytometry histograms and fluorescent images showing the SDC4 and GFP expression levels in SH-SY5Y and U-87 MG cells treated with AAV9-GFP. Scale bar = 20 μm. ( D , E ) Detected SDC4 and GFP expression levels of AAV9-treated U-87 MG cells were normalized to that of SH-SY5Y cells as standards. The bars represent the mean + SEM of three independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05; ** p < 0.01.

Journal: International Journal of Molecular Sciences

Article Title: Syndecan-4 Mediates the Cellular Entry of Adeno-Associated Virus 9

doi: 10.3390/ijms24043141

Figure Lengend Snippet: AAV9-mediated GFP transduction is more efficient in U-87 MG than in SH-SY5Y cells. WT U-87 MG and SH-SY5Y cells were incubated with AAV9-GFP (4 × 10 4 vg/cell) for 72 h. GFP expression was then analyzed with imaging flow cytometry. ( A – C ) Representative flow cytometry histograms and fluorescent images showing the SDC4 and GFP expression levels in SH-SY5Y and U-87 MG cells treated with AAV9-GFP. Scale bar = 20 μm. ( D , E ) Detected SDC4 and GFP expression levels of AAV9-treated U-87 MG cells were normalized to that of SH-SY5Y cells as standards. The bars represent the mean + SEM of three independent experiments. Statistical significance vs. standards was assessed with ANOVA. * p < 0.05; ** p < 0.01.

Article Snippet: Stable KD cells were selected in 2 mg G418 and sorted using imaging flow cytometry (Amnis® FlowSight®, Luminex Corporation, Austin, TX, USA) with APC-conjugated anti-SDC4 antibody (RnD Systems, Minneapolis, MN, USA, cat. no. FAB29181A, 5:100 dilution) and respective isotype control (rat IgG2A APC isotype control, RnD Systems, cat. no. IC006A, 5:100).

Techniques: Transduction, Incubation, Expressing, Imaging, Flow Cytometry